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- dataset description "This workflow performs differential expression analysis of RNA-seq data obtained from Oxford Nanopore long-read sequencing technology. First a transcriptome FASTA is constructed using gffread. Reads are then mapped to the transcriptome with the long-read optimized alignment tool minimap2. Next quantification is performed using salmon before normalization and differential expression analysis are conducted by PyDESeq2. The workflow can optionally analyze splice-isoforms through integrating the FLAIR workflow. Additionaly, NanoPlot is employed to analyze initial sequencing data and QualiMap is used to evaluate mapping results." assertion.
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