Matches in Nanopublications for { <http://purl.org/np/RAtAKJJbfdpICi3gBVxevjeIaangOjOsWQNHEpSpZAULw#step> ?p ?o ?g. }
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- step type Step assertion.
- step description "Count cells by haemocytometer using W.B.C. diluting fluid: Add 10 µl of cell suspension to 190 µl of W.B.C. diluting fluid and mix well. Load the cell suspension in a haemocytometer and count the cells. Adjust cell concentration at 1 x 106 cells/ml with Dulbecco's modified eagle medium supplemented with 1% of Pencillin-streptomycin solution and 10% FBS. The approximate yield of cells from 4 ml of blood varies between 107-108." assertion.
- step isStepOfPlan plan assertion.
- step precedes step assertion.
- step reagentUsed _4 assertion.
- step reagentUsed _5 assertion.
- step reagentUsed _6 assertion.
- step reagentUsed OBI_0000918 assertion.
- step labEquipmentUsed _3 assertion.
- step bioSampleUsed _1 assertion.
- step bioSampleUsed _2 assertion.