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- step type Step assertion.
- step description "After RNAse A treatment, add one volume of chloroform: isoamyl alcohol again and mix by inversions for 5 min. The tube is centrifuged for 10 min at 5000 × g at 4°C. Transfer the clear supernatant into new 2ml tube and add half volume of 5 M NaCl to the sample and mix gently by inversions." assertion.
- step reagentUsed CHEBI_26710 assertion.
- step reagentUsed _3 assertion.
- step reagentUsed _2 assertion.
- step labEquipmentUsed BAO_0010014 assertion.
- step bioSampleUsed _1 assertion.