Matches in Nanopublications for { <http://purl.org/np/RA0ckBCe4tkHarTh_j4P80KUYtsRIbvOjtKSAgrV8xYm8#step> ?p ?o ?g. }
Showing items 1 to 9 of
9
with 100 items per page.
- step type Step assertion.
- step description "After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0)." assertion.
- step isStepOfPlan plan assertion.
- step reagentUsed _5 assertion.
- step reagentUsed C62218 assertion.
- step labEquipmentUsed _4 assertion.
- step hasExperimentalOutput _1 assertion.
- step bioSampleUsed _3 assertion.
- step bioSampleUsed _2 assertion.