Nanopublications

Matches in Nanopublications for { ?s ?p ?o <https://w3id.org/np/RAghWlxHUpxu8QmKpR08vj44xBkSkeCqB7k0YSNeXIF2M/assertion>. }

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f4520e2b-26af-4854-8c6b-3870468662f3 rightsHolder "Universitet i Bergen, Institutt for biomedisin (UiB, IBM)" assertion.
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f4520e2b-26af-4854-8c6b-3870468662f3 bibliographicCitation "Frei, D. M., University of Bergen, Institute of biomedicine, Kögel, T., Hodneland, E. (2016).Raw data for Frei, D. M. et al. Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer [Data set]. Norstore. https://doi.org/10.11582/2016.00001" assertion.
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ae7a5ee5-7019-4b60-8f94-8ba310a0199a description "Contact-dependent intercellular transfer (codeIT) of cellular constituents can have functional consequences for recipient cells, such as enhanced survival and drug resistance. Pathogenic viruses, prions and bacteria can also utilize this mechanism to spread to adjacent cells and potentially evade immune detection. However, little is known about the molecular mechanism underlying this intercellular transfer process. Here, we present a novel microscopy-based screening method to identify regulators and cargo of codeIT. Single donor cells, carrying fluorescently labelled endocytic organelles or proteins, are co-cultured with excess acceptor cells. CodeIT is quantified by confocal microscopy and image analysis in 3D, preserving spatial information. An siRNA-based screening using this method revealed the involvement of several myosins and small GTPases as codeIT regulators. Our data indicates that cellular protrusions and tubular recycling endosomes are important for codeIT. We automated image acquisition and analysis to facilitate large-scale chemical and genetic screening efforts to identify key regulators of codeIT." assertion.
f4520e2b-26af-4854-8c6b-3870468662f3 description "Contact-dependent intercellular transfer (codeIT) of cellular constituents can have functional consequences for recipient cells, such as enhanced survival and drug resistance. Pathogenic viruses, prions and bacteria can also utilize this mechanism to spread to adjacent cells and potentially evade immune detection. However, little is known about the molecular mechanism underlying this intercellular transfer process. Here, we present a novel microscopy-based screening method to identify regulators and cargo of codeIT. Single donor cells, carrying fluorescently labelled endocytic organelles or proteins, are co-cultured with excess acceptor cells. CodeIT is quantified by confocal microscopy and image analysis in 3D, preserving spatial information. An siRNA-based screening using this method revealed the involvement of several myosins and small GTPases as codeIT regulators. Our data indicates that cellular protrusions and tubular recycling endosomes are important for codeIT. We automated image acquisition and analysis to facilitate large-scale chemical and genetic screening efforts to identify key regulators of codeIT." assertion.
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ae7a5ee5-7019-4b60-8f94-8ba310a0199a dateCreated "2022-03-22 00:58:15.830962+00:00" assertion.
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ae7a5ee5-7019-4b60-8f94-8ba310a0199a cite-as "Dominik Michael Frei, University of Bergen, Institute of biomedicine (UiB), Tanja Kögel, and Erlend Hodneland. "Raw data for Frei, D. M. et al. Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer." ROHub. Mar 22 ,2022. https://w3id.org/ro-id/ae7a5ee5-7019-4b60-8f94-8ba310a0199a." assertion.
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