Matches in Nanopublications for { ?s ?p ?o <https://w3id.org/np/RA_C-L7pLBKSyHDhS2YIFmqfb_-BLGOldcUb8emWvRltA/assertion>. }
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- kinetics-model type Thing assertion.
- parameter-P0 type Thing assertion.
- parameter-k-prime type Thing assertion.
- RA_C-L7pLBKSyHDhS2YIFmqfb_-BLGOldcUb8emWvRltA type AIDA-Sentence assertion.
- kinetics-model comment "Parameters P0 and k'i capture the proportion of protected RNA and digestion rate constant respectively" assertion.
- RA_C-L7pLBKSyHDhS2YIFmqfb_-BLGOldcUb8emWvRltA seeAlso method assertion.
- kinetics-model description "Mathematical model describing transcript protection dynamics with RNase gradient treatment" assertion.
- parameter-P0 description "Proportion of RNA protected from RNase degradation in the CoLoC-seq kinetics model" assertion.
- parameter-k-prime description "Pseudo-first-order rate constant describing RNA degradation velocity" assertion.
- RA_C-L7pLBKSyHDhS2YIFmqfb_-BLGOldcUb8emWvRltA text "The CoLoC-seq kinetics model uses the protected pool parameter P0 to distinguish genuinely localised organellar transcripts from surface-attached contaminants based on their degradation dynamics." assertion.
- kinetics-model SIO_000211 parameter-P0 assertion.
- kinetics-model SIO_000211 parameter-k-prime assertion.
- kinetics-model formula "f(A) = (1 - P0) * exp(-k'i * A) + P0" assertion.
- parameter-P0 SIO_000228 "0 to 1" assertion.
- parameter-P0 comment "P0=0: no protection (dissolved/disrupted membranes); P0=partial: partial protection (surface-attached); P0=1: full protection (genuine organellar localization)" assertion.
- parameter-k-prime comment "Fitted using Levenberg-Marquardt optimization; reflects differential susceptibility to RNase between transcript populations" assertion.
- RA_C-L7pLBKSyHDhS2YIFmqfb_-BLGOldcUb8emWvRltA about gkac1205 assertion.
- kinetics-model name "CoLoC-seq Kinetics Model" assertion.
- parameter-P0 name "Protected pool parameter P0" assertion.
- parameter-k-prime name "Digestion rate constant k'i" assertion.