Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RA3MmCHHYDKYpNg2eP3Tmjn2QR_wpZXHhDjJnnCsuLFY8#assertion>. }
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- step type Step assertion.
- CHEBI_8984 label "1% SDS" assertion.
- CHEBI_29234 label "1% Triton -X" assertion.
- BAO_0010014 label "1.5 ml eppendorf tube" assertion.
- CHEBI_17992 label "100mM sucrose" assertion.
- _3 label "micropistle" assertion.
- _1 label "mosquitoes" assertion.
- _2 label "mosquitoes" assertion.
- _5 label "1X STE buffer" assertion.
- _6 label "Proteinase K (20mg/ml)" assertion.
- _4 label "Rnase A (20mg/ml)" assertion.
- step description "Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it." assertion.
- step reagentUsed _5 assertion.
- step reagentUsed _4 assertion.
- step reagentUsed _6 assertion.
- step reagentUsed CHEBI_8984 assertion.
- step reagentUsed CHEBI_29234 assertion.
- step reagentUsed CHEBI_17992 assertion.
- step labEquipmentUsed BAO_0010014 assertion.
- step labEquipmentUsed _3 assertion.
- step bioSampleUsed _2 assertion.
- step hasExperimentalInput _1 assertion.